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Z652024
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Live Cell Imaging: A Laboratory Manual
| ISBN-10: | 0-87969-683-4 |
| ISBN-13: | 978-0-87969-683-2 |
Description
| General description | Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a CD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. |
Properties
| publication info | D. Spector & R. Goldman, ed., Cold Spring Harbor Laboratory Press, 2004, 450 pp., soft cover |
Table Of Contents
| Table of Contents | Section 1: Detection and Imaging Approaches to Live Cell Imaging 1. Fluorescent Protein Tracking and Detection; 2. Constructing and Expressing GFP Fusion proteins 3. Viral vectors for Introduction of GFP; 4. Gene delivery by DIrect Injection and Facilitation of Expresssion by Mechanical Stretch; 5. Microinjection of Fluorophore-labeled proteins; 6. CCD Cameras for Fluorescence Imaging of Living Cells; 7. Photobleaching techniques to Study Mobility and Molecular Dynamics of Proteins in Live Cells: FRAP, iFRAP and FLIP 8. FRET and Fluorescence Lifetime imaging Microscopy 9. Monitoring protein dynamics Using FRET based Biosensors 10. Application of Light Directed Activiation of Caged Biomolecules and CALI to problems in Cell Motility 11. Photoactivation based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus 12. Fluorescent Speckle Microscopy of Cytoskeletal Dynamics in Living Cells 13. Polarization microscopy with the LC-PolScope 14. Confocal Microscopy, Deconvolution, and Structured Illumination Methods 15. Multiphoton and Multispectral Laser-scanning Microscopy 16. Analyzing Live Cell Data and Tracking Dynamic Movements Section 2: Imaging of Live Cells and Organisms 17. In Vivo Imaging of Mammalian Cells; 18. A Sealed Preparation for Long term Ovservations of Cultured Cells 19. Live Cell Imaging of Yeast; 20. Live Imaging of C. elegans 21. Time-lapse Cinematography in Living Drosophila Tissues; 22. Single cell imaging in Animal Tumors In Vivo 23. Long term, High resolution Imaging in the Neocortex In Vivo 24. Intravital Microscopyof Normal and Diseased Tissues in the Mouse 25. Development of Mammalian Cell LInes with lac Operator Tagged Chromosomes 26. Imaging Gene Expression in Living Cells 27. Studying Mitosis in Culutred Mammalian Cells 28. Imaging Hoechst 33342 labeled Chromosomes and Fluorescent Proteins During the Cell Cycle 29. Imaging the Actin Cytoskeleton; 30. Imaging Intermediate Filament Proteins in Living Cells 31. Methods for Expressing and Analyzing GFP-Tubulin and GFP Microtubule assocaited Proteins 32. Imaging of Organelle Membrane Systems and Membrane Traffic in LIving Cells 33. Imaging Live Cells Under Mechanical Stress 34. Imaging Single Molecules Using Total Internal Reflection Fluorescence Microscopy 35. Visualization and Quantification of Single RNA molecules in Living Cells |
